Aniseh Jamshidi, Bahareh Shabanpour, Parastoo Pourashouri, Mojtaba Raeisi,
Volume 6, Issue 3 (9-2018)
Abstract
Background and Objectives: Production of fish protein hydrolysate is a method for converting the low-value economical underutilized fish species to value-added products. This study aimed to evaluate the different proportions of distilled water to the substrate on the functional characteristics, antioxidant and nutritional quality of fish protein hydrolysate of bigeye ilisha (Ilisha megaloptera) produced by enzymatic hydrolysis.
Materials and Methods: After defatting of minced fish, the hydrolysis process was carried out using three different 4:1, 5:1 and 6:1 distilled water to substrate proportions by using alcalase enzyme in three replications. The protein hydrolysate samples were analyzed for approximate composition (soluble protein, moisture, fat and ash), functional characteristics (solubility, foam capacity, and foam stability), antioxidant properties (DPPH radical scavenging activity and reducing power) and mineral composition.
Results: In this study, the ratio of distilled water to the substrate affected the protein hydrolysate properties and the highest amounts of hydrolysis degree and DPPH radical scavenging activity were observed in samples with 5:1 ratio. Fish protein hydrolysate obtained from 4:1 ratio had the highest amount of soluble protein, and no significant difference was observed in term of solubility with samples obtained by 5:1 ratio. Moreover, there were no significant differences in terms of lightness and foam capacity of samples obtained from 4:1 and 6:1 proportions.
Conclusion: According to the results of the study, using a 4:1 ratio of distilled water to substrate led to the production of fish protein hydrolysates from bigeye ilisha with higher functional properties and nutritional composition.
Farideh Kaikhosravi, Farhad Daryanoosh, Maryam Koushkie Jahromi, Javad Nemati,
Volume 8, Issue 1 (3-2020)
Abstract
Background and objective: Proper exercise and nutrition can help prevent bone disorders in old age, therefore present study aimed to investigate the effects of high intensity interval training (HIIT) with genistein (Ge) on biomechanical properties of femur bone in elderly female rats.
Material and Methods: In this experimental study, 40 elderly female rats with mean age of 18- 24 months and mean weight of 220.15±15.28 g were divided into five groups of eight rats including: 1) control (C), 2) sham (Sh), 3) HIIT, 4) HIIT + Ge, and 5) Ge. During eight weeks groups 3 and 4 performed HIIT for three sessions per week with an intensity of 90 to 95% of maximum oxygen consumption (VO2max) in high intensity intervals and 40 to 45% VO2max in low intensity intervals and groups 4 and 5 received 60 mg/kg/day Ge peritoneally. Maximum bending strength (Fmax) measured with three- point bending test and serum levels of calcium (Ca) and phosphorus were measured by o-Cresolphthalein and molybdate methods, respectively. Shapiro- Wilk, one way ANOVA with Tukey’s post- hoc tests were used for analysis of data (P≤0.05).
Results: HIIT (P=0.02), Ge (P=0.001) and HIIT+Ge (P=0.001) significantly increased Fmax also Ge (P=0.04) and HIIT+Ge (P=0.03) had more favorable effect on increasing Fmax compare to HIIT nevertheless HIIT, Ge and HIIT+Ge had not significant effect on Ca and phosphorus (P≥0.05).
Conclusion: Although HIIT and Ge alone can enhance Fmax in femur of elderly female rats, nevertheless HIIT+Ge has more favorable effect on increase of Fmax compare to HIIT.
